MaghrebDataLibMedV2, Main, Exploration, bibRecord, 000252

Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)1

Identifieur interne : 000252 ( Main/Exploration ); précédent : 000251; suivant : 000253

Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)1

Auteurs : Yasmina El Bahloul [Maroc] ; Nicolas Dauchot [Belgique] ; Ikrame Machtoun [Maroc] ; Fatima Gaboun [Maroc] ; Pierre Van Cutsem [Belgique]

Source :

Applications in Plant Sciences [ 2168-0450 ] ; 2014.

RBID : PMC:4103133

Abstract

• Premise of the study: Microsatellite loci were developed for the Moroccan endemic endangered species Argania spinosa with a combination of a typical library enrichment procedure and a 454 GS FLX Titanium–based high-throughput sequencing approach.

• Methods and Results: A genomic DNA library was enriched and further screened using (GA)₁₅, (GTA)₈, and (TTC)₈ biotin-labeled probes coupled with chemi-luminescence detection. To increase simple sequence repeat (SSR) loci number, an ultra-high-throughput sequencing-based approach was used. Evaluation of all primer pairs was performed with labeled dUTP on an ABI 3130xl sequencer. Eleven polymorphic SSR loci were selected out of 79 SSR regions and extensively characterized on 150 individuals from eight populations. Total alleles ranged from six to 19 alleles per locus while expected heterozygosity ranged from 0.618 to 0.869.

• Conclusions: The SSRs developed here will be used to further characterize the genetic diversity of A. spinosa across its distribution range, mainly in the southern part of Morocco and southwestern Algeria. They may also be transferable to other Sapotaceae species.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103133

DOI: 10.3732/apps.1300071
PubMed: 25202614
PubMed Central: 4103133

Affiliations:

Belgique, Maroc

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and characterization of microsatellite loci for the Moroccan endemic endangered species <italic>Argania spinosa</italic>
 (Sapotaceae)<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
</title>
<author><name sortKey="El Bahloul, Yasmina" sort="El Bahloul, Yasmina" uniqKey="El Bahloul Y" first="Yasmina" last="El Bahloul">Yasmina El Bahloul</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dauchot, Nicolas" sort="Dauchot, Nicolas" uniqKey="Dauchot N" first="Nicolas" last="Dauchot">Nicolas Dauchot</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur, Belgium</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Machtoun, Ikrame" sort="Machtoun, Ikrame" uniqKey="Machtoun I" first="Ikrame" last="Machtoun">Ikrame Machtoun</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gaboun, Fatima" sort="Gaboun, Fatima" uniqKey="Gaboun F" first="Fatima" last="Gaboun">Fatima Gaboun</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Van Cutsem, Pierre" sort="Van Cutsem, Pierre" uniqKey="Van Cutsem P" first="Pierre" last="Van Cutsem">Pierre Van Cutsem</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur, Belgium</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25202614</idno>
<idno type="pmc">4103133</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103133</idno>
<idno type="RBID">PMC:4103133</idno>
<idno type="doi">10.3732/apps.1300071</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000155</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000155</idno>
<idno type="wicri:Area/Pmc/Curation">000155</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000155</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000260</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000260</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:25202614</idno>
<idno type="wicri:Area/PubMed/Corpus">000563</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000563</idno>
<idno type="wicri:Area/PubMed/Curation">000561</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000561</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000584</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000584</idno>
<idno type="wicri:Area/Main/Merge">000252</idno>
<idno type="wicri:Area/Main/Curation">000252</idno>
<idno type="wicri:Area/Main/Exploration">000252</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and characterization of microsatellite loci for the Moroccan endemic endangered species <italic>Argania spinosa</italic>
 (Sapotaceae)<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
</title>
<author><name sortKey="El Bahloul, Yasmina" sort="El Bahloul, Yasmina" uniqKey="El Bahloul Y" first="Yasmina" last="El Bahloul">Yasmina El Bahloul</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dauchot, Nicolas" sort="Dauchot, Nicolas" uniqKey="Dauchot N" first="Nicolas" last="Dauchot">Nicolas Dauchot</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur, Belgium</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Machtoun, Ikrame" sort="Machtoun, Ikrame" uniqKey="Machtoun I" first="Ikrame" last="Machtoun">Ikrame Machtoun</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gaboun, Fatima" sort="Gaboun, Fatima" uniqKey="Gaboun F" first="Fatima" last="Gaboun">Fatima Gaboun</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat, Morocco</nlm:aff>
<country xml:lang="fr">Maroc</country>
<wicri:regionArea>Plant Breeding Unit Rabat Regional Research Center, National Institute of Agronomy Research, Institut National de la Recherche Agronomique (INRA) Maroc, B.P. 6570 Rabat-Instituts, 10101 Rabat</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Van Cutsem, Pierre" sort="Van Cutsem, Pierre" uniqKey="Van Cutsem P" first="Pierre" last="Van Cutsem">Pierre Van Cutsem</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur, Belgium</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Unit of Research in Plant Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Applications in Plant Sciences</title>
<idno type="eISSN">2168-0450</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>• <italic>Premise of the study:</italic>
 Microsatellite loci were developed for the Moroccan endemic endangered species <italic>Argania spinosa</italic>
 with a combination of a typical library enrichment procedure and a 454 GS FLX Titanium–based high-throughput sequencing approach.</p>
<p>• <italic>Methods and Results:</italic>
 A genomic DNA library was enriched and further screened using (GA)<sub>15</sub>
, (GTA)<sub>8</sub>
, and (TTC)<sub>8</sub>
 biotin-labeled probes coupled with chemi-luminescence detection. To increase simple sequence repeat (SSR) loci number, an ultra-high-throughput sequencing-based approach was used. Evaluation of all primer pairs was performed with labeled dUTP on an ABI 3130xl sequencer. Eleven polymorphic SSR loci were selected out of 79 SSR regions and extensively characterized on 150 individuals from eight populations. Total alleles ranged from six to 19 alleles per locus while expected heterozygosity ranged from 0.618 to 0.869.</p>
<p>• <italic>Conclusions:</italic>
 The SSRs developed here will be used to further characterize the genetic diversity of <italic>A. spinosa</italic>
 across its distribution range, mainly in the southern part of Morocco and southwestern Algeria. They may also be transferable to other Sapotaceae species.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="El Mousadik, A" uniqKey="El Mousadik A">A. El Mousadik</name>
</author>
<author><name sortKey="Petit, R J" uniqKey="Petit R">R. J. Petit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="El Mousadik, A" uniqKey="El Mousadik A">A. El Mousadik</name>
</author>
<author><name sortKey="Petit, R J" uniqKey="Petit R">R. J. Petit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glenn, T C" uniqKey="Glenn T">T. C. Glenn</name>
</author>
<author><name sortKey="Schable, N A" uniqKey="Schable N">N. A. Schable</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goudet, J" uniqKey="Goudet J">J. Goudet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaabeche, M" uniqKey="Kaabeche M">M. Kaabèche</name>
</author>
<author><name sortKey="Benkheira, A" uniqKey="Benkheira A">A. Benkheira</name>
</author>
<author><name sortKey="De Foucault, B" uniqKey="De Foucault B">B. de Foucault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Majourhat, K" uniqKey="Majourhat K">K. Majourhat</name>
</author>
<author><name sortKey="Jabbar, Y" uniqKey="Jabbar Y">Y. Jabbar</name>
</author>
<author><name sortKey="Hafidi, A" uniqKey="Hafidi A">A. Hafidi</name>
</author>
<author><name sortKey="Martinez G Mez, P" uniqKey="Martinez G Mez P">P. Martínez-Gómez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Micheneau, C" uniqKey="Micheneau C">C. Micheneau</name>
</author>
<author><name sortKey="Dauby, G" uniqKey="Dauby G">G. Dauby</name>
</author>
<author><name sortKey="Bourland, N" uniqKey="Bourland N">N. Bourland</name>
</author>
<author><name sortKey="Doucet, J L" uniqKey="Doucet J">J. L. Doucet</name>
</author>
<author><name sortKey="Hardy, O J" uniqKey="Hardy O">O. J. Hardy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Msanda, F" uniqKey="Msanda F">F. Msanda</name>
</author>
<author><name sortKey="El Aboudi, A" uniqKey="El Aboudi A">A. El Aboudi</name>
</author>
<author><name sortKey="Peltier, J P" uniqKey="Peltier J">J. P. Peltier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rozen, S" uniqKey="Rozen S">S. Rozen</name>
</author>
<author><name sortKey="Skaletsky, H" uniqKey="Skaletsky H">H. Skaletsky</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Belgique</li>
<li>Maroc</li>
</country>
</list>
<tree><country name="Maroc"><noRegion><name sortKey="El Bahloul, Yasmina" sort="El Bahloul, Yasmina" uniqKey="El Bahloul Y" first="Yasmina" last="El Bahloul">Yasmina El Bahloul</name>
</noRegion>
<name sortKey="Gaboun, Fatima" sort="Gaboun, Fatima" uniqKey="Gaboun F" first="Fatima" last="Gaboun">Fatima Gaboun</name>
<name sortKey="Machtoun, Ikrame" sort="Machtoun, Ikrame" uniqKey="Machtoun I" first="Ikrame" last="Machtoun">Ikrame Machtoun</name>
</country>
<country name="Belgique"><noRegion><name sortKey="Dauchot, Nicolas" sort="Dauchot, Nicolas" uniqKey="Dauchot N" first="Nicolas" last="Dauchot">Nicolas Dauchot</name>
</noRegion>
<name sortKey="Van Cutsem, Pierre" sort="Van Cutsem, Pierre" uniqKey="Van Cutsem P" first="Pierre" last="Van Cutsem">Pierre Van Cutsem</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000252 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000252 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    MaghrebDataLibMedV2
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4103133
   |texte=   Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)1
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25202614" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MaghrebDataLibMedV2

This area was generated with Dilib version V0.6.38.
Data generation: Wed Jun 30 18:27:05 2021. Site generation: Wed Jun 30 18:34:21 2021

Serveur sur les données et bibliothèques médicales au Maghreb (version finale)

Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)1

Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)1

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur sur les données et bibliothèques médicales au Maghreb (version finale)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.